MICROSCOPY: SCIENCE, TECHNOLOGY,

APPLICATIONS AND EDUCATION

Introduction

Application of a novel technique for observing internal ultrastructure of human chromosomes with known karyotype

Mohammad Ghazizadeh  and  Ramin Ghazizadeh

Investigation of the ultrastructure of yeast using cryo-immuno-electron microscopy

Muriel Mari, Jani10pxce Griffith and Fulvio Reggiori

Image processing in electron tomography

J.J. Fernández,  J.I. Agulleiro, J.R. Bilbao-Castro, A. Martínez, I. García, F.J. Chichón, J. Martín-Benito and  J.L. Carrascosa

Scanning electron microscopy and vascular corrosion casting for the characterization of microvascular networks in human and animal tissues

B. Minnich and A. Lametschwandtner

Use of light and electron microscopy to study neuropharmacology of neuroprotection

Hari S. Sharma, A. Sharma and José V. Lafuente

The Use of EM for Studding Apoptotic Changes and Proliferative Activity of Oral Carcinoma and Jaw Lymphoma

Professor Raja Kummoona

Morphogenesis of Human Enterovirus 71: An Electron Microscopy Analysis Coupled with Immunogold Labeling Techniques

Cai Yun CHEN, Hui Min ONG and Justin Jang Hann CHU

Microscopic studies on characterization of blood cells of endangered sea turtles

J. Orós, A. B. Casal, and A. Arencibia

Infection and phagocytosis: analysis in semen with transmission electron microscopy

Guadalupe Gallegos-Avila, Jesús Ancer-Rodríguez, Marta Ortega-Martínez and Gilberto Jaramillo-Rangel

Electron microscopy studies of genetically modified neurons and synapses

I. Nikonenko and D. Muller

In Situ Detection of Specific Gene Expression During and Immediately After Transcription at Electron Microscopic Level

S. Kitazawa, K. Mori, Y Idei, M. Fujimoto,  K. Kito, R. Haraguch, Y. Abe, T. Kondo and R. Kitazawa

Contribution of microscopy to the study of proliferating blood cells in Ciona intestinalis immune response

M. A. Di Bella and G. De Leo.

Electronic microscopy of the genus Arthrinium. A study model for the knowledge of Dematiaceous Fungi

C. Adelantado, E.L. Arosemena and M.A. Calvo

Applications of transmission electron microscopy to virus detection and identification

F.F. Vale, A.C. Correia, B. Matos, JF Moura Nunes and A.P. Alves de Matos

The antennal sensory function in the oldest pterygote insects: an ultrastructural overview

M. Rebora, S. Piersanti and E. Gaino

Advances in multilabeling for TEM and CLSM using primary antibodies derived from the same species. Application of Fab fragments to co-localize olive pollen allergens

S. Morales, MI. Rodriguez-García and J.D. Alché

A simple and reliable method to prepare semen for transmission electron microscopy

M. Relucenti, L. Petruziello, G. Familiari and R. Heyn

Scanning microscopy of ciliary organelles

Marina Silveira

The use of transmission electron microscopy to solve problems in the study of the female gonad in platyhelminths

A. Falleni, C. Ghezzani, and P. Lucchesi

Electron Microscopic Radioautography and Its Application to DNA, RNA and Protein Synthesis in Mitochondria of Various Animal Organs

T. Nagata

Application of Light and Scanning Electron Microscopy in the Identification of Herbal Medicines

R. Serrano, G. da Silva and O. Silva

Electron microscopy approaches for the investigation of the cellular targets of trypanocidal agents in Trypanosoma cruzi

M.N.C. Soeiro, A. Daliry, C.F. Silva, D.G.J. Batista, E.M. de Souza, G.M. Oliveira, K. Salomão, R.F.S. Menna-Barreto and S.L. de Castro

Application of environmental scanning electron microscopy for study of biofilms in medical devices

A. Trinidad, A. Ibáñez, D. Gómez, J.R. García-Berrocal and R. Ramírez-Camacho

Autoschizis: a new cell death induced found in tumour cells induced by oxidative stress mechanism

Jacques Gilloteaux , J.M. Jamison, D. Arnold, K. McGuire, M. Loukas, J.P. Sczepaniak and J.L. Summer

Importance of Electron Microscopy to reveal species-specific characteristics of gland secretion

Gabriella Chieffi Baccari, Alessandra Santillo and Sergio Minucci

Transmission Electron Microscopy of the nuclear matrix and its proteins

C. D’Arrigo, P. Barboro, D. Galante and C. Balbi

Using transmission electron microscopy to solve issues related with biological research in animal reproduction

L.M. Pastor, V. Seco-Rovira, E. Beltrán-Frutos, C. Ferrer, A. Zuasti, J. Pallares, A. Calvo, X. Lucas, J. Roca, J.M. Vazquez and E.A. Martínez

Application of monoclonal antibody and ultrathin cryosections in immunogold electron microscopy: a study on recombinant human inhibitor expressed in bacterial cells

T. Zavašnik-Bergant

Scanning electron microscopy of plant surfaces: simple but sophisticated methods for preparation and examination

H. J. Ensikat, P. Ditsche-Kuru and W. Barthlott

A review of SEM and TEM studies on the hybridisation of dentine

Ario Santini, Egle Milia and Vesna Miletic

Making invisible visible – Electron tomography shows a lattice surface layer in whole-mounts of Lactobacillus brevis

Peter Engelhardt, Fang Zhao, Pentti Jääskeläinen, Ritva Serimaa, Ilkka Palva and Veli-Pekka Lehto

Correlative light and electron microscopic exploration of endocytic organelles

C. Meisslitzer-Ruppitsch, T. Prikowitsch, J. Neumüller, M. Pavelka and A. Ellinger

Assessment of bacteria-textile interactions using Scanning Electron Microscopy: A study on LbL chitosan/alginate coated cotton

Ana P. Gomes, João F. Mano, João A. Queiroz and Isabel C. Gouveia

SEM image analysis as a tool for the surface characterization of pharmaceutical pellets obtained by extrusion-spheronization

L. Alonso, R. Martínez-Pacheco and J.L. Gómez-Amoza

Nissl substance and cellular structures involved in the intraneuronal and neuroglial transport in the crayfish stretch receptor

G.M.Fedorenko and A.B. Uzdensky

Morphological changes induced in bacteria as evaluated by electron microscopy

J. Díaz-Visurraga, G. Cárdenas and A. García

Localizing cellular uptake of nanomaterials in vitro by transmission electron microscopy

C.T. Ng, J.J. Li, R. Perumalsamy, F. Watt,  L.Y.L. Yung and B.H. Bay

High resolution ultrastructural investigation of insect sensory organs using field emission scanning electron microscopy

Vonnie D.C. Shields

Methodology for the characterization and identification by sem-eds of atmospheric particles from different pollutions emission

A. Campos-Ramos, A. Aragón Piña, Xavier Querol and Andrés Alastuey

SEM Study on Old-styled Thai Gold Mosaic Glass Samples

K. Won-in and P. Dararutana

Two-dimensional crystallization of membrane protein complexes for structure-function studies by electron crystallography

T.M. Dreaden, M.C. Johnson, B. Barquera, B.A. Barry and I. Schmidt-Krey

Use of transmission electron microscopy for studying airway remodeling in a chronic ovalbumin mice model of allergic asthma

G. D. Leishangthem, U. Mabalirajan, T. Ahmad,  B. Ghosh,  A. Agrawal, T. C. Nag and A. K. Dinda

Investigation of Nanostructured Organic Solar Cells with Transmission Electron Microscopy

Golap Kalita, Koichi Wakita and Masayoshi Umeno

Evaluation of morphological changes in experimental models of myocardial infarction: Electron and light microscopical evidence

Saurabh Bharti, Sachin Arora, Dharamvir S Arya

Scanning electric microscopy used to analyze the effect of gamma irradiation on enamel and dentin

C.J. Soares, C.C.G. Moura, P.B. Soares and L.Z. Naves

Unveiling 3D Biological Structures by X-ray Microtomography

 R. Mizutani,  A. Takeuchi, K. Uesugi, S. Takekoshi, R.Y. Osamura and Y. Suzuki

Use of Infrared Microspectroscopy to discriminate foodborne pathogens

S. de Lamo-Castellví and L. E. Rodríguez-Saona

X-ray in-line phase microtomography for biomedical applications

M. Langer, R. Boistel, E. Pagot, P. Cloetens, F. Peyrin

Fourier Transform Infrared (FTIR) Microspectroscopy of Immature, Mature and Degenerated Articular Cartilage

S. Saarakkala, L. Rieppo, J. Rieppo and J.S. Jurvelin

Digital X-ray microscopy of small biological samples using Medipix2 semiconductor pixel detector

J. Dammer, F. Weyda, J. Jakůbek, V. Sopko, J. Žemlička and R. Hanus

Synchrotron Radiation Infrared Microspectroscopy (SR-IRMS) of living-cells in physiological environment

Giovanni Birarda, Gianluca Grenci, and Lisa Vaccari

Atomic Force Microscopy indentation of living cells

Lucel Sirghi

Visualization of cell structure in situ by atomic force microscopy

María de L. Segura-Valdez, Alma Zamora-Cura, Nadia Gutiérrez-Quintanar, Ernesto Villalobos-Nájera, Jennifer Berenice Rodríguez-Vázquez, Tania Citlalli Galván-Arrieta, Diana Jiménez-Rodríguez, Lourdes T. Agredano-Moreno, Reyna Lara-Martínez and Luis F. Jiménez-García

The principles and biological applications of cell traction force microscopy

James H-C. Wang and Bin Li.

Detecting DNA damage at a single-molecule level by atomic force microscopy

Yong Jiang and Piotr E. Marszalek

Study of the mechanical properties of single cells as biocomposites by atomic force microscopy

M. Starodubtseva, S. Chizhik, N. Yegorenkov, I. Nikitina and E. Drozd

Force spectroscopy addresses fundamental problems in cell physiology

Hirohide Takahashi, Yaron R. Silberberg, Masahiro Kumeta, J. Michael Edwardson and Kunio Takeyasu

Atomic Force Microscopy Studies of DNA Release in Gene Delivery Dynamics

Yi Zou, Lei Wan and Guangzhao Mao

Quantitative analysis of drug induced cellular morphology using AFM

Sunil Kumar and Sohini Roy

Antibody recognition force microscopy (Ig-RFM) to identify and map the nanoscale distrubution of protein molecules on the surface of live microorganisms

Brian H. Lower

Combined AFM/Raman microspectroscopy for characterization of living cells in near physiological conditions

A.H. Zhou, G.D. McEwen and Y.Z. Wu

AFM investigations of various disturbing factors on bacterial cells

H. Nikiyan, A. Vasilchenko and D. Deryabin

Combining atomic force microscopy and live cell imaging to study calcium responses in dorsal root ganglion neurons to a locally applied mechanical stimulus

L. Ponce, A. Berquand, M. Petersen and M. Hafner

Study of a 2-branched (1->3)-b-D-glucan from Lactobacillus suebicus CUPV221 as observed by Tapping mode Atomic Force Microscopy

C. Marieta and M. T. Dueñas

Quantification of kinetics of biomembrane degradation by AFM

A. Bitler, Naama Lev and Yechiel Shai

Analysis of Surface Mechanical Properties of Unworn and Worn Silicone Hydrogel Contact Lenses Using Nanoindentation with AFM

González-Méijome JM, Almeida JB and Parafita MA

Comparison of poly (methyl methacrylate) and acrylic hydrophobic intraocular lens surface irregularities using atomic force microscopy

Koel Chaudhury, Rashmi Mukherjee and Partha Biswas

The use of confocal laser scanning microscopy in endodontic research: sealer/dentin interfaces

M. Marciano, R. Ordinola-Zapata, B. Cavalini Cavenago, A. del Carpio Perochena, C. Monteiro Bramante, I. Gomes de Moraes, R. Brandão Garcia, P. Gagliardi Minotti and M. Hungaro Duarte

Review: Upconversion microscopy for biological applications

D.H. Kim and J.U. Kang

The use of confocal laser scanning microscopy for the study of dentin infection

R. Ordinola-Zapata, C. Monteiro Bramante, I. Gomes de Moraes, N. Bernardineli, V. Carvalho Porto, AP. Campanelli, RB Garcia and M. Húngaro Duarte

Confocal scanning laser microscopy in the study of biofilm formation in tissues of the upper airway in otolaryngologic diseases

Paraje María Gabriela

In vivo oral biofilm analysis by confocal laser scanning microscopy: Methodological approaches

Tomás I, Henderson B, Diz P and Donos N

Taxonomical discrimination of pollen grains by using confocal laser scanning microscopy (CLSM) imaging of autofluorescence

A.J. Castro, J.D. Rejón, M. Fendri, M.J. Jiménez-Quesada, A. Zafra, J.C. Jiménez-López, M.I. Rodríguez-García and J.D. Alché

Application of Confocal Microscopy for Quantification of Intracellular Mycobacteria in Macrophages

P. Bettencourt, D. Pires, N. Carmo and E. Anes

Imaging In vitro Anti-biofilm Activity to Visualize the Ultrastructural Changes

S. Sasidharan, L. Yoga Latha and T. Angeline

Advanced approaches of confocal microscopy to study interactions of membrane active peptides with living cells: hemolytic activity of latarcin Ltc1

O.V. Samsonova, A.S. Arseniev,  M.P. Kirpichnikov and A.V. Feofanov

Towards In Vivo microimaging of Lung Inflammation and Repair: Current issues and future directions

O. Lesur, P.G. Charette, Ph Jouvet, F. Cheriet and F. Chagnon

Epifluorescence, confocal laser microscopy and colocalization analyses in the study of protein glutathionylation in primary cultured fibroblasts and in their in situ extracted matrix

Stefania Petrini, Fiorella Piemonte, Chiara Passarelli and Enrico Bertini

Two and three dimensional imaging in confocal laser scanning microscopy - application for collagen VI defect studies

Stefania Petrini, Adele D’Amico, Teresa Rizza, Marianna Coccetti, Rosalba Carrozzo,  Francesca Gualandi and Enrico Bertini

Breaking down the genome organization and karyotype differentiation through the epifluorescence microscope lens: insects and fishes as models

D. C. Cabral-de-Mello and C. Martins

Study of molecular dynamics using fluorescently tagged molecules in live cells

C. N. Goulbourne, A. N. Malhas, H. Doolittle, A. T. Ismail and D. J. Vaux

High resolution three-dimensional imaging of membrane junctional protein dysfunction using deconvolution microscopy

D. Segretain, J. Gilleron, J. Dompierre, D. Carette, J.-P. Denizot and G. Pointis

Variations of the spatial fluorescence distribution in ABL gene chromatin domains measured in blood cell nuclei by SMI microscopy after COMBO – FISH labelling

C. Grossmann, J. Schwarz-Finsterle, E. Schmitt, U. Birk, G. Hildenbrand, C. Cremer, L. Trakhtenbrot and M. Hausmann

Quantitative and sensitive live-cell imaging with FIDSAM (fluorescence intensity decay shape analysis microscopy) for biomaterials research

F. Schleifenbaum,  A.-K. Born, S. Peter and K. Maniura-Weber

Fluorescence Deconvolution Microscopy and cellular modeling to understand heart repair after ventricular unloading

Roger J. Bick, Brian J. Poindexter, Stephanie T. Bates, Courtney J. Gemmato, O. H. Frazier and Pippa M. Evans

Quantum dots in microscopy and cytometry: immunostaining applications

N. Barteneva and I.Vorobjev

Nanofabricated DNA Curtains for High-throughput Single-Molecule Imaging of Protein-DNA Interactions

H. N. Wolcott, B. Alcott, L. Kaplan and E. C. Greene

The adapted “single-section” Golgi method and the DiI fluorescent dye in microscopy for describing neuronal and glial morphology in different species

Alberto A. Rasia-Filho, Aline Dall’Oglio, Francine Dalpian, Liliam Midori Ide, Janaína Brusco and Jorge E. Moreira

Single molecule fluorescence imaging of receptor interactions in mammalian cell signalling

David T. Clarke, Daniel Rolfe, Stephen E.D. Webb, Martyn Winn and Marisa L. Martin-Fernandez

Bimolecular fluorescence complementation (BiFC) to investigate composition and cellular localization of homo- and heterodimeric transmembrane receptors and intracellular signalling pathways

T. Förg, L. Ponce, A.Tomsche, A. Holloschi, A. Lux and M. Hafner

Use of fluorescence photobleaching techniques to measure the kinetics of intracellular transport

R.G. Davies, D.A. Jans and K.M. Wagstaff

Live-Cell Fluorescent Imaging of Membrane or Mitochondrion Transfer between Connected Cells in Culture

A. Cselenyák, E. Pankotai, A. Csordás, L. Kiss and Z. Lacza

Analysis of Molecular Mobility by Fluorescence Recovery After Photobleaching in Living Cells

C. Klein and F. Waharte